Tag
Part:BBa_K4392002:Design
Designed by: Vikram Venugopal Group: iGEM22_IISER-Pune-India (2022-10-01)
6x Histidine Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the part belonged to the pHis17 plasmid backbone we used, we did not have to deal with any special design considerations.
Source
This part was sourced from the pHis17 plasmid backbone, onto which restriction-free cloning was done.